ANNOUNCEMENTS
The aim of this work was to optimise and investigate the mass production system for the variations in cultures arising due to differences in subculture and harvesting time frames at Centre for Mycorrhizal Research, Bioresources and Biotechnology Division, TERI. For this, Agrobacterium-mediated transformation was carried out for various plant explants to obtain hairy roots. The symbiosis variations of these new host cultures along with the existing root hosts was tested with 10 different arbuscular mycorrhizal fungus to screen for the variations in the sporulation pattern, root biomass, colonisation percentage and viability. Further, an insight in the biochemical and molecular basis of symbiosis was carried out. For the biochemical analysis, nutrient analysis for C, N, P, K was studied across different hosts in Glomus intraradices over a time frame of 6 weeks and 12 weeks. For the molecular analysis, the new and the existing root lines were confirmed for the transformation event, the root diversity analysis was carried out by AFLP and real time expression study for the alkaline phosphate gene was studied using Glomus intraradices with 3 different hosts to ascertain aspects of the symbionts under in vitro conditions. As a new strategy, EMROC was established to compare the production by the conventional in vitro soilrite-vermiculite methods and the root-host-based method. For the formulation of the product, lyophilisation was carried out as a technique to increase the shelf life of the formulated product.
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