ANNOUNCEMENTS
Cancer is a polyetiological and multifactorial disease that can develop through one of numerous pathways. Its diverse nature often makes it difficult to provide timely diagnosis and successful treatment. One of the routes through which cancer develops and progresses is through the alternative or aberrant splicing of certain genes, leading to the formation of various isoforms that are associated with different subtypes of the disease. Studying the conditions under which these splicing events are regulated in the cells can help us better understand the origin and development of cancer.
In this study we generated plasmids to study splicing events in two ways. The first objective was to study the gene regulation of an RNA binding protein, through the generation of transcription factor- binding site mutants. The second was the development of a dual luciferase reporter construct that would allow us to observe both MEAS and skipping events during splicing. These plasmids can be utilized to study splicing events under specific conditions in several genes across various cancer subtypes.
Keywords: Cancer, Splicing, Luciferase, Plasmids, Mutant.
